AN IMMOBILIZED ANTIBODY-BASED AFFINITY GRID STRATEGY FOR ON-GRID PURIFICATION OF TARGET PROTEINS ENABLES HIGH-RESOLUTION CRYO-EM

An immobilized antibody-based affinity grid strategy for on-grid purification of target proteins enables high-resolution cryo-EM

Abstract In cryo-electron microscopy (cryo-EM), sample preparation poses a critical bottleneck, particularly for rare or fragile macromolecular assemblies and those suffering from denaturation and particle orientation distribution issues related to air-water interface.In this study, we develop and characterize an immobilized antibody-based affinity

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Subclinical Lipopolysaccharide from Salmonella Enteritidis Induces Dysregulation of Bioactive Substances from Selected Brain Sections and Glands of Neuroendocrine Axes

Bacterial lipopolysaccharide (LPS) can contribute to the pathogenesis and the clinical symptoms of many diseases such as cancer, mental disorders, neurodegenerative as well as metabolic diseases.The asymptomatic copyright state of Salmonella spp.is Saddle a very important public health problem.A subclinical single dose of LPS obtained from S.Enteri

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Update on Practical Management of Early-Stage Non-Small Cell Lung Cancer (NSCLC): A Report from the Ontario Forum

Therapeutic strategies for early-stage non-small cell lung cancer (NSCLC) are advancing, with immune checkpoint inhibitors (ICIs) and targeted therapies making their way into neoadjuvant and adjuvant settings.With recent advances, there was a need for multidisciplinary lung cancer healthcare providers from across Ontario to convene and review recen

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